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Influence Of Different Nitrogen Sources On Growth And Phb Production Of Bacterial Isolates
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Polyhydroxybutyrate (PHB) is ne of the polyhydroxyalkanoates (PHAs) which has biodegradable and biocompatible properties. Samples were collected form groundnut farm garden in Tsaragi at share in Kwara State. A total of twenty one (21) bacteria were isolated form the soil sample in which bacillus sp were 12 in number while the % frequency of occurrence is (54.5%). Veillococcus sp is 3 and the % frequency of occurrence is (9.1%)s, enterococcus sp, corynebacterium sp, Yersinia Pesis, Lactobacillus fermenta and citrobacter sp were all 1 in number and there % frequency of occurrence is (4.5%) each. The isolates were screened for PHB production using Sudan III stain as well as submerged fermentation. Four (4) of the best PHB producing bacterial were selected for PHBs production.
The fermentation pattern of PHB production was studies using Nitrogen source. NH4Cl (1.560a) was found to be the best effect of PHB growth producing bacteria i.e. (citrobacter sp) and KNO3 (1.899a) the highest for PHB production.
The studies carried out shown that nitrogen sources influence the production of PHB i.e. (citrobacter sp).
 
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TABLE OF CONTENTS - [ Total Page(s): 1 ]TABLE OF CONTENTTitle pageCertificationDedicationAcknowledgementAbstractTable of contentCHAPTER ONE1.0 Introduction1.1 Aims and objectives1.2 Statement of problem1.3 JustificationCHAPTER TWO2.0 Literature reviewCHAPTER THREE3.0 Materials and method3.1 Preparation of media3.2 Isolation of bacterial from soil samples3.3 Maintenance of culture3.4 Identification of bacterial isolates3.4.1 Morphological characterization3.4.2 Biochemical characterization3.5 Screening o isolate for PHB production using ... Continue reading---
CHAPTER ONE - [ Total Page(s): 2 ]The bacterium capable of producing polyhydroxybutyrate has been identified in more than twenty (20) bacterial genera, including Azatobacter, Bacillus< Beijernickia, Pseudomonas. Alcaligenes, Rhizobium and Rhodospirillum (Sudesh et al., 2000).many researchers have explained that soil bacteria generally produce polyhydroxybutrate,polyhydroxybutyrate PHB) production increases if ambient conditions (ph, temperature, nutrients) are made available (Hanzlikova et al., 1985).Pollyhydroxyalkanoate is one ... Continue reading---
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TABLE OF CONTENTS - [ Total Page(s): 1 ]TABLE OF CONTENTTitle pageCertificationDedicationAcknowledgementAbstractTable of contentCHAPTER ONE1.0 Introduction1.1 Aims and objectives1.2 Statement of problem1.3 JustificationCHAPTER TWO2.0 Literature reviewCHAPTER THREE3.0 Materials and method3.1 Preparation of media3.2 Isolation of bacterial from soil samples3.3 Maintenance of culture3.4 Identification of bacterial isolates3.4.1 Morphological characterization3.4.2 Biochemical characterization3.5 Screening o isolate for PHB production using ... Continue reading---
ABSRACT -- [Total Page(s) 1]
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ABSRACT -- [Total Page(s) 1]
Page 1 of 1