3.4.4 HEAVY METALS
Digestion of water sample
• 10mls of the sample was pipetted into soul digestion tubes
• 5mls of HNO3 was added the tube was mounted on the preheated digestion block in a fume cupboard
• It was digested for hours
• It was allowed to cool to warm temperature
• It was made to 50mls digestion volume with ultrapure water.
• It was covered with paraffin paper transporter
• It was swirled for 2 minutes
• It was transferred to a set of centrifuge tubes
• It was shaked for 10 minutes on a mechanical shaker
• It was later centrifugal for 5 minutes at 5rpm
• The supernatant was transferred to a set of plastic vials for determination of the metals on AAS
• The AAS (Atomic Absorption Spectrophotometer) was on and calibrated for a particular element in sequential order.
• The samples table was prepared i. e the sample I.D standard concentration, digestion volume and dilution factor
• The standard absorbance was run first, followed by the sample of uniform concentration at their volume wavelength slit, size.
3.5 Research Method
The study was carried out using observational descriptive research design
3.6 Sampling Technique
This study was sampled using simple random sampling.
3.7 Statistical Analysis
The statistical analysis was done with SPSS version 16.o software package and descriptive analysis was done using Microsoft excel software (Microsoft office, 2010). The results were expressed using simple descriptive statistic to analyse the data and assess the results of ANOVA and DUNCAN MULTIPLE TEST statistical tools. Difference was considered statistically significant at p ˂0.05.