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The Antiplasmodial Activity Of Extracts Of Edible Mushroom: Agaricus Bisporus On Plasmodium Berghei In Albino Mice
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1.4 Aim and Objectives
1.4.1 Aim
To determine the antiplasmodial activity of extracts of edible mushroom: Agaricus bisporus on Plasmodium berghei in albino mice.
1.4.2 Objectives
The specific objectives of this study were to:
a. assess the analytical components of edible mushroom (Agaricus bisporus) using Gas Chromatography Mass Spectrophotometry (GCMS).
b. determine the antiplasmodial activity of edible mushroom extract: (Agaricus bisporus) on Plasmodium berghei.
c. determine the effect of mushroom extract on the temperature and weight of mice infected with Plasmodium berghei.
d. compare the effect of aqueous and alcoholic mushroom extract on malaria parasitemia.
1.5 Research Hypotheses (Null)
a. There is no significant difference in the analytical components of Agaricus bisporus extract and a known standard drug for Plasmodium berghei.
b. There is no significant difference in the antiplasmodial activity of Agaricus bisporus extract on Plasmodium berghei and a known standard drug for Plasmodium berghei.
c. Agaricus bisporus extract has no significant difference in effect on the temperature and weight of mice infected with Plasmodium berghei.
d. Aqueous and alcoholic Agaricus bisporus extract have no significant difference on malaria parasitemia.
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ABSRACT - [ Total Page(s): 1 ]ABSTRACT IS COMING SOON ... Continue reading---
CHAPTER TWO - [ Total Page(s): 4 ]HPLC-based activity profiling and subsequent chromatography of the ethyl acetate extract of Ganoderma lucidum yielded six lanostanes (106–112) of which three (107, 108, 112) were new (Fig. 1). These lanostanes exhibited moderate in vitro antiplasmodial activity with IC50 values of 6 to 20 Μm (Adams et al., 2010). Investigation of the chemical constituents of fungus, Cordyceps nipponica BCC 1389 led to the identification of four isolates of N-hydroxy- and N-methoxy-2-pyridones compounds ... Continue reading---
CHAPTER THREE - [ Total Page(s): 2 ]CHAPTER THREE3.0 MATERIALS AND METHODS3.1 Sample Collection Sufficient mushrooms (Agaricus bisporus) was gotten from Igunsin village, Akure north in Ondo State, Nigeria and taken to the Herbarium Service Unit (HSU), Department of Plant Biology, University of Ilorin, Ilorin, Kwara State, Nigeria for authentication with a voucher number of F.H.I. 11295.3.2 Drying and ExtractionThe mushroom samples were cut into pieces using a sharp knife. The pieces were air-dried properly ... Continue reading---
CHAPTER FOUR - [ Total Page(s): 6 ]CHAPTER FOUR4.0 RESULTSThe compounds present in the aqueous and alcoholic extract of Agaricus bisporus were identified by GC-MS analysis after analysis. Aqueous mushroom extract was labelled as A while the alcoholic extract was labelled B. The active principle Molecular Weight (MW), Concentration (%), Molecular Formula (MF), and Retention Time (RT). Nine compounds were identified in the extracts. The prevailing compounds in the aqueous extract were 1-Butanamine, 2-methyl-N- (2-methylbtylid ... Continue reading---
CHAPTER FIVE - [ Total Page(s): 2 ]CHAPTER FIVE5.0 DiscussionThis result of the Gas Chromatograghy Mass Spectrophotometry identified the compounds present in the fruiting body of A. bisporus. The prevailing compounds in the aqueous extract were 1-Butanamine, 2-methyl-N- (2-methylbtylidene) (2.03%), 2-Pyrorolidinone (7.46%) while the prevailing compounds in the alcoholic extract were n-Hexadecanoic acid (19.47%) and 9,12-Octadecadienoic acid (Z,Z) (80.53%). According to Isaka et al. (2001), these are some of the active ingre ... Continue reading---
REFRENCES - [ Total Page(s): 2 ]REFERENCESAdams, M., Christen, M., Plitzko, I., Zimmermann, S., Brun, R., Kaiser, M., Hamburger, M. (2010) Antiplasmodial lanostanes from the Ganoderma lucidum mushroom. Journal of Natural Products, 73:897–900.Akindahunsi, A. A., and Oyetayo, F. L. (2012). Nutrient and anti-nutrient distribution of edible mushroom, Pleurotus tuberregium (Fries) Singer. Food Science and Technology, 39(5):548-553.Anthony, M.P., Burrows, J.N., Duparc, S., Moehrle, J.J.,Wells, T.N.C. (2012) The global pipelin ... Continue reading---
