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The Antiplasmodial Activity Of Extracts Of Edible Mushroom: Agaricus Bisporus On Plasmodium Berghei In Albino Mice
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The result showed that group 5 (the group infected and treated with chloroquine) recorded the highest percentage suppression of 82.97% at the end of the experiment. Among the treated groups, the group given 200mg/ml of the ethanolic extract of the mushroom recorded the least percentage suppression of 69.87%. Comparatively, the hot water extract exerted a higher percentage suppression of 78.17% than the ethanolic extract which exerted 77.73% at 400mg/ml; thereby making the hot water extract more potent than the ethanolic extract (Table 4).
The result of parasitemia count and the percentage suppression exerted by the mushroom extracts on the mice after 48 hours. The result showed that group 5 (the group infected and treated with chloroquine) recorded the highest percentage suppression of 98.48% at end of the experiment. Among the treated groups, the group given 200mg/ml of the ethanolic extract of the mushroom recorded the least percentage suppression of 77.44%. Comparatively, the hot water extract exerted a higher percentage suppression of 93.60% than the ethanolic extract which exerted 84.18% at 400mg/ml; thereby making the hot water extract more potent than the ethanolic extract (Table 5).
The result of parasitemia count and the percentage suppression exerted by the mushroom extracts on the mice after 72 hours showed that group 5 (the group infected and treated with chloroquine) recorded the highest percentage suppression of 99.80% with only two parasites was seen on the smear at end of the experiment. Among the treated groups, the group given 200mg/ml of the ethanolic extract of the mushroom recorded the least percentage suppression of 80.16%. Comparatively, the hot water extract exerted a higher percentage suppression of 96.84% than the ethanolic extract which exerted 90.92% at 400mg/ml; thereby making the hot water extract more potent than the ethanolic extract (Table 6).
The result of parasitemia count and the percentage suppression exerted by the mushroom extracts on the mice after 96 hours showed that no parasite was seen at all for the group infected and treated with chloroquine. Among the treated groups, the group given 200mg/ml of the ethanolic extract of the mushroom recorded the least percentage suppression of 80.57%. Comparatively, the hot water extract exerted a higher percentage suppression of 99.64% than the ethanolic extract which exerted 96.90% at 400mg/ml; also making the hot water extract more potent than the ethanolic extract (Table 7).
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ABSRACT - [ Total Page(s): 1 ]ABSTRACT IS COMING SOON ... Continue reading---
CHAPTER ONE - [ Total Page(s): 2 ]1.4 Aim and Objectives1.4.1 AimTo determine the antiplasmodial activity of extracts of edible mushroom: Agaricus bisporus on Plasmodium berghei in albino mice.1.4.2 ObjectivesThe specific objectives of this study were to:a. assess the analytical components of edible mushroom (Agaricus bisporus) using Gas Chromatography Mass Spectrophotometry (GCMS).b. determine the antiplasmodial activity of edible mushroom extract: (Agaricus bisporus) on Plasmodium berghei.c. ... Continue reading---
CHAPTER TWO - [ Total Page(s): 4 ]HPLC-based activity profiling and subsequent chromatography of the ethyl acetate extract of Ganoderma lucidum yielded six lanostanes (106–112) of which three (107, 108, 112) were new (Fig. 1). These lanostanes exhibited moderate in vitro antiplasmodial activity with IC50 values of 6 to 20 Μm (Adams et al., 2010). Investigation of the chemical constituents of fungus, Cordyceps nipponica BCC 1389 led to the identification of four isolates of N-hydroxy- and N-methoxy-2-pyridones compounds ... Continue reading---
CHAPTER THREE - [ Total Page(s): 2 ]CHAPTER THREE3.0 MATERIALS AND METHODS3.1 Sample Collection Sufficient mushrooms (Agaricus bisporus) was gotten from Igunsin village, Akure north in Ondo State, Nigeria and taken to the Herbarium Service Unit (HSU), Department of Plant Biology, University of Ilorin, Ilorin, Kwara State, Nigeria for authentication with a voucher number of F.H.I. 11295.3.2 Drying and ExtractionThe mushroom samples were cut into pieces using a sharp knife. The pieces were air-dried properly ... Continue reading---
CHAPTER FIVE - [ Total Page(s): 2 ]CHAPTER FIVE5.0 DiscussionThis result of the Gas Chromatograghy Mass Spectrophotometry identified the compounds present in the fruiting body of A. bisporus. The prevailing compounds in the aqueous extract were 1-Butanamine, 2-methyl-N- (2-methylbtylidene) (2.03%), 2-Pyrorolidinone (7.46%) while the prevailing compounds in the alcoholic extract were n-Hexadecanoic acid (19.47%) and 9,12-Octadecadienoic acid (Z,Z) (80.53%). According to Isaka et al. (2001), these are some of the active ingre ... Continue reading---
REFRENCES - [ Total Page(s): 2 ]REFERENCESAdams, M., Christen, M., Plitzko, I., Zimmermann, S., Brun, R., Kaiser, M., Hamburger, M. (2010) Antiplasmodial lanostanes from the Ganoderma lucidum mushroom. Journal of Natural Products, 73:897–900.Akindahunsi, A. A., and Oyetayo, F. L. (2012). Nutrient and anti-nutrient distribution of edible mushroom, Pleurotus tuberregium (Fries) Singer. Food Science and Technology, 39(5):548-553.Anthony, M.P., Burrows, J.N., Duparc, S., Moehrle, J.J.,Wells, T.N.C. (2012) The global pipelin ... Continue reading---
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