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Sub-chronic Effect Of Co-administration Of Methformine And Amilodipine On Some Haematological Indices In Experimental Animal
[A CASE STUDY OF WISTAR RATS]
REFRENCES -- [Total Page(s) 4]
Page 3 of 4
-
-
-
Liu, K.W., Dai, L.K. and Jean, W. (2006). metformin-related vitamin B12 deficiency. Age and Ageing. 35(2): 200-201.
Majumder, S., Soriano, J., Cruz, A.L. and Dasanu, C.A. (2013). Vitamin B-12 deficiency in patients undergoing bariatric surgery: Preventive strategies and key recommendations. Surgery for Obesity and Related Diseases. 9(6):1013-1019.
Maida, A., Lamont, B.J., Cao, X. and Drucker, D.J. (2011). Metformin regulates the incretin receptor axis via a pathway dependent on peroxisome proliferator-activated receptor-α in mice. Diabetologia. 54: 339-349.
Mazokopakis, E.E. and Starakis, I. K. (2012). Recommendations for diagnosis and management of metformin-induced vitamin B12 (Cbl) deficiency. Diabetes Research and Clinical Practice. 97(3): 359-367.
McGovern, L., Johnson, J.N., Paulo, R., Hettinger, A. and Singhal, V. (2008). Clinical review: treatment of pediatric obesity: a systematic review and meta-analysis of randomized trials. Journal of Clinical Endocrinology and Metabolism. 93: 4600-4605.
Miller, R.A., Chu, Q., Xie, J., Foretz, M., Viollet, B. (2013). Biguanides suppress hepatic glucagon signalling by decreasing production of cyclic AMP. Nature. 494: 256-260.
Montville, J.B., Ahuja, J.C., Martin, C.L., Heendeniya, K.Y., Omolewa-Tomobi, G., Steinfeldt, L.C. and Moshfegh, A. (2013). USDA Food and Nutrient Database for Dietary Studies (FNDDS), 5.0. In P. Stumbo & S. McNutt (Eds.). 36th National Nutrient Databank Conference. Amsterdam: Elsevier Science Bv. Pp 99-112.
Musarrat, K., Kalathil, D. and Varughese, G.I. (2008). Metformin, B12 and homocysteine levels: The plausible cause or effect?. Journal of the Formosan Medical Association. 107(6): 505-506.
Nathan, D.M., Buse, J.B., Davidson, M.B., Ferrannini, E. and Holman, R.R. (2009) Medical management of hyperglycemia in type 2 diabetes: a consensus algorithm for the initiation and adjustment of therapy: a consensus statement of the American Diabetes Association and the European Association for the Study of Diabetes. Diabetes Care. 32: 193-203.
Nervo, M., Lubini, A., Raimundo, F.V., Faulhaber, G.M., Leite, C., Fischer, L.M. and Furlanetto, T.W. (2011). Vitamin B12 in metformin-treated diabetic patients: a crosssectional study in Brazil. Revista Da Associacao Medica Brasileira. 57(1): 46-49.
Ou, H.Y., Cheng, J.T., Yu, E.H. and Wu, T.J. (2006). Metformin increases insulin sensitivity and plasma beta-endorphin in human subjects. Hormonal Metabolism Research. 38: 106-111.
Obeid, R., Jung, J., Falk, J., Herrmann, W., Geisel, J., Friesenhahn-Ochs, B. and Kostopoulos, P. (2013). Serum vitamin B12 not reflecting vitamin B12 status in patients with type 2 diabetes. Biochimie. 95(5): 1056-1061.
Owen, M.R., Doran, E., Halestrap, A.P. (2000). Evidence that metformin exerts its anti-diabetic
effects through inhibition of complex 1 of the mitochondrial respiratory chain. Biochemical Journal. 348(3): 607-614.
Park, M.H., Kinra, S., Ward, K.J., White, B. and Viner, R.M. (2009). Metformin for obesity in children and adolescents: a systematic review. Diabetes Care. 32: 1743-1745.
Patade, G.R. and Marita, A.R. (2014). Metformin: A Journey from countryside to the bedside. Journal of Obessity and Metabolism Research. 1: 127-130.
Pernicova, I. and Korbonits, M. (2014). Metformin--mode of action and clinical implications for diabetes and cancer. Nature Review Endocrinology. 10: 143-156.
Quinn, S.M., Baur, L.A., Garnett, S.P. and Cowell, C.T. (2010). Treatment of clinical insulin resistance in children: a systematic review. Obesity Review. 11: 722-730.
Ribeiro-Oliveira A., Nogueira, A.I. and Pereira, R.M. (2008). The renin-angiotensin system and diabetes: an update. Vascular Health and Risk Management Journal. 4(4): 787-803.
Rojas, L.B. and Gomes, M.B. (2013). Metformin: an old but still the best treatment for type 2 diabetes. Diabetology Metabolism and Syndrome Journal. 5: 6.
Rosenbloom, A.L., Silverstein, J.H., Amemiya, S., Zeitler, P. and Klingensmith, G.J. (2009). Type 2 diabetes in children and adolescents. Pediatric Diabetes Journal. 10(12): 17-32.
Saeedi, R., Parsons, H.L., Wambolt, R.B., Paulson, K. and Sharma, V. (2008). Metabolic actions of metformin in the heart can occur by AMPK-independent mechanisms. American Journal of Physiology Heart Circulation Physiology. 294: 2497-2506.
Salpeter, S., Greyber, E., Pasternak, G. and Salpeter, E. (2006). Risk of fatal and nonfatal lactic acidosis with metformin use in type 2 diabetes mellitus. Cochrane Database Systemic Review Journal. 67: 100-110.
REFRENCES -- [Total Page(s) 4]
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ABSRACT - [ Total Page(s): 1 ]ABSTRACTMetformin, which belongs to the biguanide class, is one of the most generally used oral hypoglycemic agents. It has been used for more than 50 years and was approved by the US Food and Drug Administration (FDA) in 1994 (American Diabetes Association, 2009) whereas Amlodipine is a long acting dihydropyridine calcium channel blocker, which is used in the treatment of angina to lower the BP (Blood pressure). the aim is to know the effect of co-administration of this two drugs in Wistar rats ... Continue reading---
APPENDIX A - [ Total Page(s): 1 ]APPENDIXAPPENDIX 1: MATERIAL USED1% Ammonium oxalate10% Formalin salineAbsorbent paperAutomatic pipetteBrilliant cresyl blueCounting chamber Cyanmethaemoglobin standardDrabkin’s ReagentEDTA sample containerHematoxylin and Eosin stainKhan tubes/rackLeisfhmann stainLight microscopeMicrohaematocrit centrifugeNormal salinePasteur pipettePipette tipsRotary microtomeSpectrophotometerTimer Turk’s solution Universal sample bottle ... Continue reading---
APPENDIX B - [ Total Page(s): 1 ]APPENDIX IITable 1: haematological parameters in control, Metformine treated and Amilodipine treated Rats (Values are expressed as mean ± Standard error of means) Group A(1): control (untreated), Group B(2): co-administration of Metformin 0.00264/ml/132g and Amlodipine 0.000849/ml/132g at a single dose for 30 daysPARAMETERS GROUP A GROUP BPacked cell volume (%) 44.16±2.83 38.47±3.53Red blood cell count (x1012/L) 7 ... Continue reading---
LIST OF TABLES - [ Total Page(s): 1 ]LIST OF TABLESTable 1: Haematological parameters in control, Co-administration of Metformin and Amlodipine treated ... Continue reading---
LIST OF FIGURES - [ Total Page(s): 1 ]LIST OF FIGURESFigure 1: Shows mechanism of action of metformin Figure 2: Shows Pie chat representation of Packed cell volume of control, Co-administration of metformin and amlodipine treated Wistar Rats Figure 3: Shows Histogram representation of Hemoglobin and red blood cell count of control, Co-administration of metformin and amlodipine treated Wistar Rats Figure 4: Shows Histogram representation of red cell indicies of control, Co-administration of metformin and amlodipine t ... Continue reading---
TABLE OF CONTENTS - [ Total Page(s): 1 ]TABLE OF CONTENTTitle page Declaration Certification Dedication Acknowledgement Table of content List of table List of figures Abstract CHAPTER ONE1.0 Introduction 1.1 Background of study 1.2 Statement of Problems 1.3 Justifications 1.4 Aims 1.5 Research Objectives 1.6 Research Hypothesis 1.7 Significance of research CHAPTER TWO2.0 Literature review 2.1 Metformin 2.1.1 ... Continue reading---
CHAPTER ONE - [ Total Page(s): 2 ]CHAPTER ONE1.0 INTRODUCTION1.1 Background of the studyMetformin, which belongs to the biguanide class, is one of the most generally used oral hypoglycemic agents. It has been used for more than 50 years and was approved by the US Food and Drug Administration (FDA) in 1994 (American Diabetes Association, 2009). Currently, many clinical practice guidelines for patients with type 2 diabetes, including the American Diabetes Association (ADA), the European Association for the Study of Diabetes ... Continue reading---
CHAPTER TWO - [ Total Page(s): 12 ]The current use of serum Vitamin B12 level relies on several different range points for Vitamin B12 status. Serum Vitamin B12 level under 148 pmol/L are said to be deficient, low/borderline levels are interpreted from levels between 148-220 pmol/L (Mazokopakis and Starakis, 2012). Normal ranges of Vitamin B12 serum range from 220- 800 pmol/L, levels that exceed this are rarely suspicious in cause, although excessive B12 serum may be a marker of myloproliferative disorders (Pernicova and Korbonit ... Continue reading---
CHAPTER THREE - [ Total Page(s): 4 ]From the above table,Km of rats= 6 Km of human= 37For Amlodipine, Human dose= 10mg/70kg 1kg=10/70 =0.14mg/kgAED of Amilodipine= 0.14×(6/37) =0.02mg/kgAverage weight of experimental Animal is 132g(0.132kg)If 0.02mg of Amlodipine is administered per kg.bw,(0.02×0.132)mg of Amlodipine was administered to ... Continue reading---
CHAPTER FOUR - [ Total Page(s): 6 ]CHAPTER FOUR4.0 Results4.1 Hematological parameter in control and test groups As summarized in table 1, the mean RBC counts were 7.62 ± 2.83 x1012/L for the control and 6.53 ± 0.57 x1012/L for co-administration of metformin and amlodipine fed groups respectively. Although, decrease in RBC counts was observed in the treated (co-administration of metformin and amlodipine) dose group, these were statistically significant compared to control groups (student t test P0.05) higher in the tr ... Continue reading---
CHAPTER FIVE - [ Total Page(s): 2 ]CHAPTER FIVE5.0 DiscussionMetformin (1,1-dimethylbiguanide) is the most widely used drug to treat type 2 diabetes, and is one of only two oral anti-diabetic drugs on the World Health Organization (WHO) list of essential medicines (American Diabetes Association, 2009). Amlodipine is a long acting dihydropyridine calcium channel blocker, which is used in the treatment of angina to lower the BP. Co-administration of Metformin and Amlodipine have been discovered to lead to vitamin B12 deficiency. Al ... Continue reading---
