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Evaluating The Levels Of Some Selected Trace Metals, Renal Function Parameters And Liver Function Parameters Of Heavy Cosmetics Users Among Female Undergraduates And Compare Levels With Non-cosmetic Users
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ALBUMIN
Pipette into test tubes
Reagent standard sample
Distilled water 0.01ml --- ----
Standard (CAL) --- 0.01ml ---
Sample --- --- 0.01ml
BCG reagent (R1) 3.00ml 3.00ml3.00ml
It was mixed and incubated for 5minute at room temperature and the absorbance was read spectrophotometrically at 630nm against a reagent blank. 
Contents concentration of solutions
R1. Biuret reagent
Sodium hydroxide 100mmol/l
Na-K-tartarate 16mmol/l
Potassium iodide 15mmol/l
Cupric sulphate 6mmol/l
R2. Blank reagent
Sodium hydroxide 100mmol/l
Na-K-t
Artarate 16mmol/l
CAL. standard
Protein 59.89g/l or 5.99g/dl
 
Pipette into Cuvette
Reagent standard sample
Distilled water 0.02ml -- --
Standard (CAL) -- 0.02ml --
Serum -- -- 0.02ml
R1 1.0ml 1.0ml1.0ml
It was mixed and incubated for 30minutes at room temperature and the absorbance was read spectrophotometrically at 546nm against a reagent blank.
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ABSRACT - [ Total Page(s): 1 ]Abstract is coming soon ... Continue reading---
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ABSRACT - [ Total Page(s): 1 ]Abstract is coming soon ... Continue reading---