Antimicrobial Effects Of Three Species Of Mangifera Indica

MATERIALS AND METHOD

2.0 Plant Materials

Three varieties of Mangifera indica leaves were gotten from Nsukka area, Enugu state. The leaves were from species locally known as -Kerosene mango, -German mango, and -Sheri mango (sweet mango), respectively. The Sheri mango produces fruits smaller than the first two.

2.1 Preparation of plant materials

The leaves were washed for dust with tap water and rinsed with distilled water. They were dried under shade (at room temperature) for 10 days. After drying, the leaves were crushed into a powdered form with a sterile grinding machine. The powdered form was stored in an airtight container.

2.2 Test organisms

Laboratory isolates of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli were gotten and sub-cultured in nutrient agar and then kept in the incubator for 24hrs when they will be used.

2.3 Extraction

The plant product was extracted using distilled water and ethanol. For the aqueous extract 25g of the Mangifera leaf powder was poured into 250ml of water, and left to stand for 72 hours. The filtrates were gotten using a sterile filter paper. For the ethanolic extract, 10g of the Mangifera leaf powder was poured into 100ml of ethanol and kept for 48 hours before being filtered with a sterile filter paper. This was done for the three species in both cases.

2.4 Microbiological media

 The media used for this work was: Mueller Hinton agar (for the susceptibility testing) and nutrient agar (for sub-culturing the bacteria).

2.5 Assay for the antimicrobial activity

This was carried out using agar well diffusion method. The petri-dishes are filled with Mueller Hinton agar. The test organisms is each adjusted to 0.5 McFarland standards and inoculated onto the agar plates using a sterile swab stick. Two concentrations of the extracts were made in both extraction solvents 0.1ml (the initial concentration) and 0.01ml, two wells were made in each plate using a sterile cork borer (6mm in size) the two concentrations were added to the wells. The plates were left to stand on the working table for thirty minutes before the plates were incubated. The control was done using commercially available gentamicin a standard antibiotic.