• Isolation Of Micro-organisms From Stored Pap

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    • 1.2     STATEMENT OF PROBLEM
      Microorganisms are involved in the processing of pap especially during fermentation and equally during storage.  Few organisms are found in the pap, if it is properly stored.  Their presence in pap during storage leads to irregular of flavour and loss of nutrients (van veen and steinkrans 1990).  This is the result of their metabolic activity in the stored pap.
                The tradiitonal method of pap production using various grains encourage significant nutrient losses, Losses m,ay occur during steeping, milling and sieving. Large parts of the protein in the grain is located in the testa and germ that are shifted off during processing.
                Losses of fibre, protein, ash as well as some vitamins have been reported by Banigo and Muller (1972). Losses in nutrients could be minimized by using an improved wet milling method devised by Banigo and Muller (1972).  At the end of this work, the microorganisms found in stored pap was isolated and characterized.
      1.3     AIM AND OBJECTIVES OF THE STDU
      The aim of this study is to isolate and characterize micro-organisms from stored pap.
      SPECIFIC OBJECTIVES ARE
      i.                   To isolate and characterize bacterial contaminants from stored pap
      ii.                 To isolate and characterize fungal contaminants from stored pap
      1.4     HYPOTHESIS
                HI:    Bacteria and fungi are involved in the fermentation and storage of maize pap.
      1.5     JUSTIFICATION OF THE STUDY
      Not very much work has been done in this in recent times, with reference to the microbiology of the process.  Some of the earliest workdone in this include those of Akiurele (1970-1977) and Barigo (1969, 1970, 1972, 1977).
      1.6     SIGNIFICANCE OF STUDY
      Pap stored improperly for a relatively length of time is likely to develop microorganisms.  Therefore, the study is carried-out to identify this organisms and properly advice on the proper way of storing pap.
      1.7     LIMITATION OF THE STUDY
      This work is limited to viable microorganism present in the corn (zea mays) only which will be purchased from Ogbete in the Enugu Area.
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