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Isolation And Identification Of Microorganisms From Herbal Mixtures Sold At Enugu Metropolis
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The safety, efficacy and quality of herbal mixtures have been an important concern for health authorities and health professional, especially now there is increase in the use of herbal mixtures.
This study was aimed at isolation and identification of microorganisms from some liquid herbal mixtures sold in Enugu metropolis, South East of Nigeria. A total of twenty samples of herbal mixture were selected at random, from herbal shops in Enugu metropolis and were analysed in Microbiology laboratory, Godfery Okoye University, samples were inoculated onto Nutrient agar, MaCkonkey agar and Blood agar plates, and incubated at 37oC for 24 hrs.
Potatoe dextrose agar slants were also inoculated for the isolation of fungi. Viable bacterial counts of the samples were also performed using nutrient agar. The organisms isolated were identified using biochemical tests, and the following organisms were identified Streptococcus sp, Escherichia coli, Staphylococcus sp, Proteus sp, Aspergillus sp, Rhizopus sp, Pseudomonas sp Bacillus sp Penicillum sp mucor sp. the herbal medicine were highly contaminated and most of the organisms, isolated were gotten through poor handling, poor manufacturing of this herbal mixtures and raw materials used in preparing this herbal mixtures.
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LIST OF TABLES - [ Total Page(s): 1 ]TABLE ONE: COLONIES MORPHOLOGY OF THE ORGANISM ISOLATED ON NUTRIENT AGAR TABLE TWO: COLONIES MORPHOLOGY OF THE ORGANISM ISOLATED ON MACKONKEY AGARTABLE FOUR: VIABLE PLATE COUNTTABLE FIVE: COLONIES MORPHOLOGY OF THE ORGANISM ISOLATED ON POTATOE DEXTROSE AGAR TABLE SIX: GRAMS STAINING TABLE SEVEN: BIOCHEMICAL TABLE ... Continue reading---
TABLE OF CONTENTS - [ Total Page(s): 1 ]TITLE:TITLE PAGE APPROVAL PAGEDEDICATION ACKNOWLEDGEMENT TABLE OF CONTENTSLIST OF TABLESABSTRACTCHAPTER ONE: INTRODUCTION 1.0 INTRODUCTION 1.1 BACKGROUND OF STUDY 1.2 AIM 1.3 OBJECTIVE CHAPTER TWO: 2.0 LITERATURE REVIEW2.1 ORGISINSM ISOLATED FROM HERBAL PREPARATION 2.2 HISTROY OF HERBAL MEDICINES 2.3 SOME HERBAL MEDICINE IN USE IN NIGERIA AND THEIR APPLICATION 2.4 W H O APPROVAL FOR HERBAL MEDICINE2.5 COMMON MICROBIAL CONTAMINAT ASSOCIATED WITH MEDICINAL PLAN ... Continue reading---
CHAPTER ONE - [ Total Page(s): 1 ]1.0 INTRODUCTION1.1 BACKGROUND OF STUDYHerbal medicines are naturally occurring plant derived substances that are used to treat illness with local or regional healing practices. And these products are complex mixtures of organic chemicals that may come from any raw or processed part of a plant.Herbal medicine botanically is known as medicine or phytomedicine, is it the process of using plant seeds, Berries roots, leaves, barks, or flower for medicinal purposes, which many of them are believed to ... Continue reading---
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LIST OF TABLES - [ Total Page(s): 1 ]TABLE ONE: COLONIES MORPHOLOGY OF THE ORGANISM ISOLATED ON NUTRIENT AGAR TABLE TWO: COLONIES MORPHOLOGY OF THE ORGANISM ISOLATED ON MACKONKEY AGARTABLE FOUR: VIABLE PLATE COUNTTABLE FIVE: COLONIES MORPHOLOGY OF THE ORGANISM ISOLATED ON POTATOE DEXTROSE AGAR TABLE SIX: GRAMS STAINING TABLE SEVEN: BIOCHEMICAL TABLE ... Continue reading---
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