• The Effects Of Metformin And Diabinese On Female Sex Hormone Of Type 2 Diabetes Mellitus Patients
    [UNIVERSITY OF ILORIN TEACHING HOSPITAL (UITH), ILORIN, KWARA STATE]

  • APPENDIX A -- [Total Page(s) 1]

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    • APPENDIX I
      QUESTIONNAIRE TO ACCESS THE ANTHROPOLOGIC AND DEMOGRAPHIC INDICES OF PATIENTS WITH TYPE TWO DIABETES MELLITUS ON ANTIDIABETIC DRUGS (METFORMIN AND DIABINESE) ATTENDING UITH ILORIN.
      INTRODUCTION: I am a final year students of the Department of Medical Laboratory Science, School of Basic Medical Sciences, Kwara State University, Malete, Kwara State. This questionnaire is aimed at accessing the demographic indices of patients with type 2 diabetes mellitus on metformin and diabinese in Ilorin metropolis, Kwara State.All information provided here will be treated with utmost confidentiality and be utilized for the purpose of this study.
      Please kindly help complete the questionnaire, your name is not compulsory. Tick appropriate boxes and thanks for your anticipation.
      Identification number
      INSTRUCTION: please tick in the appropriate box
      1. Name----------------------------------------------------------- (Optional)
      2. Sample Code----------------------------------------------------------
      3. Age ------------------------------------------------------years
      4. Occupational status----------------------------------------------------------
      5. Average monthly income----------------------------------------------------------
      6. State/ Town of Origin: ----------------------------------------------------------
      7. Marital status: Single               Married
          If married, how many number of children-------------------------------------------------------
      8. Level of formal Education:  None          Primary             Secondary            Tertiary
      9. Do you Smoke:    Yes          No
           If yes, name the cigarettes/per day you smoke: -----------------------------------------------
      10. Do you drink Alcohol?  Yes            No
           If yes, what brand? Beer OR Alcoholic wine --------------------------------------------------
        How many bottles per day? ----------------------------------------------------------
      11. Height --------------------cm
      12. Weight---------------------kg
      13. You have diabetes mellitus?   Yes             No
      14. How did  you know before coming to the hospital that you have diabetes mellitus -----------------------------------------------------------------------------------------------------------------
      15. Age when you are diagnosed of diabetes mellitus----------------------------------------years
      16. Any family history of diabetes? Yes                 No     
      17. If yes, what is the relationship between you and the family member --------------------------
      18. How often do you test your blood sugar? ----------------------------------------------------------
      19. Are you on antidiabetic drugs?     Yes            No
      20. If yes which type? METFORMIN            DIABINESE              Others
      21. How long have you been taking the pills?. --------------------------------------------------------
      22. Do you have any other medical challenge(s)? Yes              No                                                                                                      
           If yes, does it include any of the following? Hypertension           ulcer            Heart disease   
            Kidney disease            others
      23. Are you on special diet recommended by Dietician?  Yes             No
      24. If yes, what are the types of Food, list them.1---------------------- 2-----------------------
             3---------------------- 4 ----------------------------5----------------------------
      25. State the combination of food you eat at a time-----------------------------------------------
             Thanks for your cooperation, God bless.
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    • ABSRACT - [ Total Page(s): 1 ]ABSTRACT is coming soon ... Continue reading---

         

      APPENDIX B - [ Total Page(s): 1 ]APPENDIX 11REAGENT COMPOSITION FOR ESTROGENEstrogen calibrates, Horseradish Peroxidase Conjugates, Estrogen biotinylated purified rabbit igG conjugates, Tetremethylbenzidine(TMB),  wash buffer concentrate, assay buffer, Stop solution.REAGENT COMPOSITION FOR PROGESTERONEProgesterone calibrates, Horseradish Peroxidase Conjugates, Progetarone biotinylated purified rabbit igG conjugates, Tetremethylbenzidine (TMB),  wash buffer concentrate, assay buffer, Stop solution. ... Continue reading---

         

      CHAPTER ONE - [ Total Page(s): 2 ]CHAPTER ONEINTRODUCTION1.1    BACKGROUND OF STUDY Diabetes mellitus is a group of metabolic disorders in which a person has high plasma glucose, either because the body does not produce enough insulin, or because cells do not respond to the insulin that is produced. The high plasma glucose produces the classical symptoms of polyuria, polydipsia and polyphagia (Rother, 2007). Type 2 diabetes mellitus, formerly non-insulin dependent diabetes mellitus or adult onset diabetes, is a ... Continue reading---

         

      CHAPTER TWO - [ Total Page(s): 17 ]CHAPTER THREE3.0                 MATERIALS AND METHODS3.1        STUDY AREA    The study was conducted in the Metropolitan City of Ilorin, Kwara State. Ilorin, the capital city of Kwara State, Nigeria is located on latitude 8o 24’N and 8o 36’N and longitude 4o 10’E and 4o 36’E with an area of about 100Km2 (Kwara State Diary, 1997). 3.2 STUDY POPULATIONThe population of this study was centred on patients with type 2 diabetes mellitus on anti-diab ... Continue reading---

         

      CHAPTER THREE - [ Total Page(s): 6 ]3.7.2 REAGENT    Materials provided with the kit: 1.    Antibody-Coated Wells (1 plate, 96 wells) Microtiter wells coated with 1.0 µg/ml streptavidin was packaged in an aluminium.2.    Reference Standard Set (1ml/vial) Contains 0, 20, 100, 250, 500  1500,3000 in pg/ml with preservatives.3.    Rabbit Anti-estrogen Reagent (6 ml) Contains rabbit anti-estradiol biotinylated rabbit igG conjugate in buffer, yellow dye with preservatives.4.    Estradiol enzyme reagent (6 m ... Continue reading---

         

      CHAPTER FOUR - [ Total Page(s): 3 ]Table 4.4: Comparison of biochemical parameters (estrogen, progesterone and fasting blood sugar) between diabetic patients on metformin only and diabetic patients on combined metformin with other drugs (glimepride).Table 4.5 depicts the Pearson correlation of duration of diabetes and BMI with biochemical parameters (estrogen, progesterone and fasting blood sugar) in diabetic patients using antidiabetic drugs (metformin and diabinese).Data indicated a significant correlation between biochemical p ... Continue reading---

         

      CHAPTER FIVE - [ Total Page(s): 2 ]CHAPTER FIVE5.0    DISCUSSIONType 2 diabetes mellitus, formerly known as non-insulin dependent diabetes mellitus or adult onset diabetes, is a metabolic disorder that is characterized by hyperglycemia in the context of insulin resistance and relative insulin deficiency (Vinay et al., 2008). Sex differences and the role of gonadal hormones in modulating insulin sensitivity and glucose tolerance are of increasing interest and importance because of the increasing prevalence of type 2 diabetes me ... Continue reading---

         

      REFRENCES - [ Total Page(s): 4 ]Murali, R. and Saravanan, R. (2012). Antidiabetic effect of d-limonene, a monoterpene in streptozotocin-induced diabetic rats. Biomedical Prevention and Nutrition.2:269-275.Neerati, P., Devde, R., and Gangi, A.K. (2014). Evaluation of the effect of curcumin capsules on glyburide therapy in patients with type-2 diabetes mellitus. Phytotherapeutic. Research. 28:1796-1800.Nelson, L.R. and Bulun, S.E. (2011).Estrogen production and action. Journal of America Academic and Dermatology. 4 ... Continue reading---