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The Effects Of Metformin And Diabinese On Female Sex Hormone Of Type 2 Diabetes Mellitus Patients
[UNIVERSITY OF ILORIN TEACHING HOSPITAL (UITH), ILORIN, KWARA STATE] -
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CHAPTER THREE
3.0 MATERIALS AND METHODS
3.1 STUDY AREA
The study was conducted in the Metropolitan City of Ilorin, Kwara State. Ilorin, the capital city of Kwara State, Nigeria is located on latitude 8o 24’N and 8o 36’N and longitude 4o 10’E and 4o 36’E with an area of about 100Km2 (Kwara State Diary, 1997).
3.2 STUDY POPULATION
The population of this study was centred on patients with type 2 diabetes mellitus on anti-diabetic drugs metformin and diabinese attending University of Ilorin Teaching Hospital (UITH) Ilorin. Also apparently healthy female subject of same age range were recruited from Ilorin metropolis to serve as control. Informed consent was obtained from each participant after proper notification and information on the nature of the research, risk involved, benefits as well as confidentiality.
3.3 INCLUSION CRITERIA
Only subjects that have been quarried for type II diabetes mellitus and on either of these antidiabetes drugs metformin and diabinese for at least one year were recruited for this study.
3.4 EXCLUSION CRITERIA
Subjects that are not type II diabetes mellitus and are not on either of these antidiabetic drugs metformn and diabinese were not recruited for this study.
3.5 SAMPLE SIZE DETERMINATION
The sample size for this study was obtained using the formula described by (Daniel, 1999).
The sample size for this study was determined based on three factors:
1. The estimated prevalence of variable interest from literature review.
2. Confidence interval of 95%.
3. The acceptable margin of error.
The sample size will was then calculated according to the following formula:
n = required sample size
Z = confidence level at 95% (standard value of 1.96)
P = estimated prevalence of type 2 diabetes mellitus in Nigeria (6.8%) (Ogbera and Ekpebegh, 2014).
m = margin of error at 5% (standard value=0.05)
Thus,
Therefore, the sample size of the study was increased to one hundred (100) which will encompass sixty (60) subjects with type 2 diabetes mellitus and forty (40) non-diabetic subjects to serve as control.
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ABSRACT - [ Total Page(s): 1 ]ABSTRACT is coming soon ... Continue reading---
APPENDIX A - [ Total Page(s): 1 ]APPENDIX IQUESTIONNAIRE TO ACCESS THE ANTHROPOLOGIC AND DEMOGRAPHIC INDICES OF PATIENTS WITH TYPE TWO DIABETES MELLITUS ON ANTIDIABETIC DRUGS (METFORMIN AND DIABINESE) ATTENDING UITH ILORIN.INTRODUCTION: I am a final year students of the Department of Medical Laboratory Science, School of Basic Medical Sciences, Kwara State University, Malete, Kwara State. This questionnaire is aimed at accessing the demographic indices of patients with type 2 diabetes mellitus on metformin and diabinese in Ilor ... Continue reading---
APPENDIX B - [ Total Page(s): 1 ]APPENDIX 11REAGENT COMPOSITION FOR ESTROGENEstrogen calibrates, Horseradish Peroxidase Conjugates, Estrogen biotinylated purified rabbit igG conjugates, Tetremethylbenzidine(TMB), wash buffer concentrate, assay buffer, Stop solution.REAGENT COMPOSITION FOR PROGESTERONEProgesterone calibrates, Horseradish Peroxidase Conjugates, Progetarone biotinylated purified rabbit igG conjugates, Tetremethylbenzidine (TMB), wash buffer concentrate, assay buffer, Stop solution. ... Continue reading---
CHAPTER ONE - [ Total Page(s): 2 ]CHAPTER ONEINTRODUCTION1.1 BACKGROUND OF STUDY Diabetes mellitus is a group of metabolic disorders in which a person has high plasma glucose, either because the body does not produce enough insulin, or because cells do not respond to the insulin that is produced. The high plasma glucose produces the classical symptoms of polyuria, polydipsia and polyphagia (Rother, 2007). Type 2 diabetes mellitus, formerly non-insulin dependent diabetes mellitus or adult onset diabetes, is a ... Continue reading---
CHAPTER TWO - [ Total Page(s): 17 ]CHAPTER THREE3.0 MATERIALS AND METHODS3.1 STUDY AREA The study was conducted in the Metropolitan City of Ilorin, Kwara State. Ilorin, the capital city of Kwara State, Nigeria is located on latitude 8o 24’N and 8o 36’N and longitude 4o 10’E and 4o 36’E with an area of about 100Km2 (Kwara State Diary, 1997). 3.2 STUDY POPULATIONThe population of this study was centred on patients with type 2 diabetes mellitus on anti-diab ... Continue reading---
CHAPTER FOUR - [ Total Page(s): 3 ]Table 4.4: Comparison of biochemical parameters (estrogen, progesterone and fasting blood sugar) between diabetic patients on metformin only and diabetic patients on combined metformin with other drugs (glimepride).Table 4.5 depicts the Pearson correlation of duration of diabetes and BMI with biochemical parameters (estrogen, progesterone and fasting blood sugar) in diabetic patients using antidiabetic drugs (metformin and diabinese).Data indicated a significant correlation between biochemical p ... Continue reading---
CHAPTER FIVE - [ Total Page(s): 2 ]CHAPTER FIVE5.0 DISCUSSIONType 2 diabetes mellitus, formerly known as non-insulin dependent diabetes mellitus or adult onset diabetes, is a metabolic disorder that is characterized by hyperglycemia in the context of insulin resistance and relative insulin deficiency (Vinay et al., 2008). Sex differences and the role of gonadal hormones in modulating insulin sensitivity and glucose tolerance are of increasing interest and importance because of the increasing prevalence of type 2 diabetes me ... Continue reading---
REFRENCES - [ Total Page(s): 4 ]Murali, R. and Saravanan, R. (2012). Antidiabetic effect of d-limonene, a monoterpene in streptozotocin-induced diabetic rats. Biomedical Prevention and Nutrition.2:269-275.Neerati, P., Devde, R., and Gangi, A.K. (2014). Evaluation of the effect of curcumin capsules on glyburide therapy in patients with type-2 diabetes mellitus. Phytotherapeutic. Research. 28:1796-1800.Nelson, L.R. and Bulun, S.E. (2011).Estrogen production and action. Journal of America Academic and Dermatology. 4 ... Continue reading---
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ABSRACT - [ Total Page(s): 1 ]ABSTRACT is coming soon ... Continue reading---