The Prevalence Of Malaria Seropositive And Seronegative Population Among Kwara State University
[A CASE STUDY OF MALETE STUDENTS.]

3.3 ELIGIBILITY CRITERIA
Inclusion criteria
1.    All students from different college were included in the study
2.    All student positive for malaria parasite infection by peripheral smear were also included in the study.
3.    For control apparently healthy male and female within the age limits without any form of ailment are included.
Exclusive criteria
1.    Students from any college having any other co-infections like enteric fever, dengue fever, sepsis, UTI, meningitis, encephalitis or any form ailment visible to the eye e.g rashes and also from the questionnaire apart from malaria infection are excluded from the study.
3.4 Collection of sample
All subjects that participated in the study was bleed 2-5mls of venous blood collected into EDTA bottle for both control subject and test subject (purple cap bottle). The samples was spun using a centrifuge at 3000g for 5 minutes and the plasma was obtained by separating it into a clean plain bottle. The plasma obtained will then be used for the determination of seropositve and seronegative students.
3.5 Laboratory analysis of samples
Detection of the seropositivity or seronegativity of each student was done using rapid diagnostic test strip (RDT) kits used for antibody detection of malaria parasite. Microscopic examination of malaria parasite was done using thick and thin film to determine which specie of Plasmodium is common among KWASU students.
Title: Rapid antibody malaria diagnostic kit for malaria infection (Plasmodium falciparum)
Principle
    The malaria P.f test contains a membrane strip which is pre-coated with monoclonal antibody specific to HRP-II & pLDH of the plasmodium falciparum species on test band (P.f). The Malaria antibody colloidal gold conjugate and sample moves along the membrane chromatographically to the test region (P.f) and forms a visible line as the antibody-antigen Malaria antibody gold particle complex forms with high degree of sensitivity and specificity. This test device has a letter of pf and C as “Test Line” and “Control Line” on the surface of the case.Both the Test Line (Pf) and Control Line in result window are not visible before applying any samples. The Control Line is used for procedural control. Control line should always appear if the test is performed properly.
Procedure:
Collection using a lancet :
a) The kit and samples was brought down to Room Temperature.
b) The device (cassette) was removed from the pouch just prior to testing.
c) The device was then placed on a flat surface.
d) With the aid of a 20μl disposable Inverted cup, the inverted cup of a loop was dipped into the blood specimen and carefully placed into the round sample window.
e) 3 drops of buffer was dropped into the buffer window.
f) The results was read between 3-30 minutes & if background is not cleared add 1 more
drop of diluent. DO NOT READ ANY RESULT BEYOND TWENTY
MINUTES.
g) Any Line Appearing After 20 Minutes Would Be Of No Diagnostic Value.