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The Prevalence Of Malaria Seropositive And Seronegative Population Among Kwara State University
[A CASE STUDY OF MALETE STUDENTS.]
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Microscopy
Preparation of Thick Blood Films
A
drop of blood of each patients sample was placed at the centre of a
clean microscope slide. The cover of each syringe needle was used to
spread the blood drop in a circular motion to approximately a diameter
of 0.4cm.The slides were allowed to air dry (Cheesbrough, 2006).
Preparation of 5% Giemsa in Phosphate Buffer (pH 7.2)
5gram
of Giemsa powder was weighed using a weighing balance.0.41 gram of
Na2HPO4 and 0.65 gram of KH2PO4 was also weighed and dissolved in 100ml
of distilled water. After dissolution of the solute, 5gram of Giemsa was
added to the mixture, shaken properly and allowed to stand for 24hours.
Staining of the Thick Blood Films
The
dried unfixed thick blood films samples were stained using 5% Giemsa
solution in phosphate buffer (pH 7.2). The slides were allowed to stand
for 20 minutes after which they were washed using tap water (without
streaming). After washing, the slides were kept in an upright position
to air dry (Cheesbrough, 2006).
Examination of the Stained Thick blood films slides
A
drop of oil immersion was placed on the Giemsa stained thick blood
films and mounted on the microscope. This was examined using objective
X100 lens after focusing. The malaria parasites seen were identified and
a grading scheme of (+) =10 parasites, (++) = 11-20, (+++) = more 30
parasites was used to establish the levels of parasitaemia.
3.6 Statistical analysis
Data
was checked for errors and completeness before entry into Data base
where by summary statistics sheet was analysed using SPSS statistical
software, version 16.0 and graphs generated using Excel Spreadsheet
Software (Microsoft Office, 2010). Bivariate and multivariate logistic
regression analysis was employed to examine the association between
socio demographic variables and other risk factors with malaria
prevalence, and the Factors associated with malaria infection was
generated from this regression. Association between proportions of the
KWASU University student who we tested positive and those who we tested
negative was compared using Chi–square. A p-value of less than 0.05 was
considered significant.
3.7 Ethical consideration and informed consent
In
line with Helsinki Declaration, approval for the study was obtained
from the Health Research Ethics Committee of Kwara State Ministry of
Health. The participants were informed on the objectives, benefits and
procedure of the study and they were assured of confidentiality,
voluntariness and protection. Each participant was identified with a
unique number. A written Informed consent was obtained from each of the
participant before enrolment into the study. The investigations were
carried out at no cost to the participants.
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ABSRACT - [ Total Page(s): 1 ]ABSTRACT HERE ... Continue reading---
CHAPTER ONE - [ Total Page(s): 3 ]CHAPTER ONE 1.0 INTRODUCTION 1.1. Background of the studyMalaria is a potentially deadly disease characterized by cyclical bouts of fever with muscle stiffness, shaking and sweating (WHO, 2000). Macleod (1998) also stated that malaria is a parasitic infection transmitted to humans through the bites of an infected female Anopheles mosquito. The name “malaria†is derived from the Italian words Mal (bad) and aria (air). It arose originally because the citizens of Rome thought that the d ... Continue reading---
CHAPTER TWO - [ Total Page(s): 6 ]The two methods in routine use are light microscopy and rapid diagnostic tests (RTDs) (Giribaldi et al., 2004; WHO, 2008).a.
Light microscopic test: this is the direct microscopic visualization of
the parasite on the thick and thin blood smear of patients (WHO, 2010).
However, the risk of false negative microscopy is higher if the patient
has received a recent dose of an artemisinin derivative (Gobbi et al.,
2005). Microscopy can be used for speciation and quantification of
parasites ... Continue reading---
CHAPTER FOUR - [ Total Page(s): 4 ]Hypothesis Two: The There is no significant difference in malaria seropositive individual among Kwara State University Students on the basis of academic status.Table 4.2 shows the calculated chi-square value of 1.98 at p > 0.05. Since the p-value is greater than 0.05, the null hypothesis which states that there is no significant difference in malaria seropositive individual among Kwara State University Students on the basis of academic status is accepted. This means that there is no significant ... Continue reading---
CHAPTER FIVE - [ Total Page(s): 1 ]CHAPTER FIVE5.0 DISCUSSION, CONCLUSION AND RECOMMENDATIONSThis study determined the prevalence of malaria seropositive and seronegative population among Kwara State University, Malete students. Related literature review was made considering scholars explanation of the subject matter. Relevant data for the study was generated through laboratory experiment conducted by the researcher. Two research hypotheses were formulated and the hypotheses stated that (1) there is no significant difference in m ... Continue reading---
REFRENCES - [ Total Page(s): 4 ]REFERENCESAbdel-Gadir, A.M. (2015). Dynamics of Drug-Resistant Plasmodium falciparum in Areas of Seasonal Malaria Transmission in Sudan. Journal of Sudan Biological Science. 2(2):23-29. Abdul-Raheem, I.S. and Parakoy, D.B. (2009). Factors affecting mothers’ health care seeking behaviour for childhood illnesses in a rural Nigerian setting. Early Child Development and Care. 179(5): 671 – 683.Aderamo, A. J. (2007). Transport and Socioeconomic Development in Kwara State, Nigeria. The N ... Continue reading---
